LOCUS       pLV-[hsa-miR-299]        9117 bp    DNA     circular     30-APR-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7038..7898
                     /label=Amp(R)
     promoter        8099..8639
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5643..5877
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6452..6907)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5949..6079
                     /label=SV40 pA
     misc_feature    6080..6293
                     /label=SV40 ori
     misc_feature    4964..5560
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3582
                     /label=intron
     CDS             3629..4954
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3372
                     /label=hsa-mir-299 precursor
BASE COUNT     2161 a      2381 c      2471 g      2104 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatcct cttctcagcg 
     3121 tcaaatgcag cctagaagaa gcgccctcag gcaacttcgt ggacatgagc cttcttccag 
     3181 ccaccaccac caccatcgtg cggtacttga agaaatggtt taccgtccca catacatttt 
     3241 gaatatgtat gtgggatggt aaaccgcttc ttggtatcca gcccagccat ccaaacctcc 
     3301 ctggagaaag cctggagtta gaggagagcc acgggtctcg gcatccgcca tgccatggca 
     3361 cttggtggaa tggctagctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg 
     3421 ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg 
     3481 cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc 
     3541 aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg aaaactaccc 
     3601 ctctagagtc gagctaccgg tcgccaccat ggtgagcaag ggcgaggagg ataacatggc 
     3661 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga 
     3721 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct 
     3781 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat 
     3841 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc 
     3901 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac 
     3961 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg 
     4021 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc 
     4081 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa 
     4141 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc 
     4201 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga 
     4261 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat 
     4321 ggacgagctg tacaaggacc caccggtcgc caccatgacc gagtacaagc ccacggtgcg 
     4381 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga 
     4441 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct 
     4501 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga 
     4561 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc 
     4621 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat 
     4681 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg 
     4741 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga 
     4801 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc 
     4861 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gagtgcccga aggaccgcgc 
     4921 gacctggtgc atgacccgca agcccggtgc ctgagcggcc gcgtcgacaa tcaacctctg 
     4981 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 
     5041 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 
     5101 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 
     5161 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 
     5221 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 
     5281 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 
     5341 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc 
     5401 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 
     5461 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct 
     5521 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattcgagc tcggtacctt 
     5581 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg 
     5641 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt 
     5701 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc 
     5761 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg 
     5821 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta 
     5881 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga 
     5941 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 
     6001 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 
     6061 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct 
     6121 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc 
     6181 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg 
     6241 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc 
     6301 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca 
     6361 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc 
     6421 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg 
     6481 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 
     6541 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 
     6601 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 
     6661 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 
     6721 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 
     6781 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg 
     6841 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt 
     6901 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct 
     6961 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat 
     7021 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg 
     7081 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg 
     7141 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc 
     7201 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat 
     7261 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact 
     7321 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca 
     7381 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact 
     7441 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg 
     7501 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg 
     7561 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg 
     7621 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg 
     7681 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag 
     7741 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc 
     7801 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga 
     7861 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat 
     7921 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc 
     7981 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 
     8041 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 
     8101 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 
     8161 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc 
     8221 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 
     8281 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 
     8341 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 
     8401 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 
     8461 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 
     8521 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 
     8581 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 
     8641 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 
     8701 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta 
     8761 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag 
     8821 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga 
     8881 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg 
     8941 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg 
     9001 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc 
     9061 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgc 
//
